Journal: Molecular ecology resources
Article Title: Applying genomic data in wildlife monitoring: development guidelines for genotyping degraded samples with reduced single nucleotide polymorphism (SNP) panels
doi: 10.1111/1755-0998.13136
Figure Lengend Snippet: Boxplots display the counts of errors or missing data over sample sets across all scorable SNP loci. The bars within boxplots represent medians, while data points show single samples that appear as outliers. (A) Error rates - allelic dropouts (blue) and false alleles (red). (B) Rates of missing data resulting from invalidation of whole SNP loci or whole sample genotypes during scoring (orange) or lack of genotype signal for single samples at single loci, e.g. no calls (grey). (C) Number of loci that produced scorable genotype data (light grey, numbers) or failed in genotyping (dark grey). 1 n = 92 reference samples (high-DNA, Table 1) with known genotypes; 2 n = 90 duplicated tissue samples from various European wildcat populations; 3 n = 30 triplicated hair samples from genetic wildcat monitoring in Germany; single failing marker was later successfully substituted for the final panel.
Article Snippet: 96.96 Dynamic Array TM SNP genotyping SNP genotyping was performed on 96.96 Dynamic ArraysTM (Fluidigm) with integrated fluidic circuits (IFCs, Wang et al., 2009 ).
Techniques: Produced, Marker